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ATCC human embryonic kidney hek293 cells

MiR-136-3p content in cell culture medium and uptake of extracellular miR-136-3p into cultured myotubes. MiRNA content in (A) human myotubes and (B) human pancreatic islets culture media. Results were first normalized using RNU1A1 and then presented in relation to miR-23a-3p content for n = 4 different donors for myotubes cultures and n = 4 donors for human islets. Left panel (C) shows bright-field image of cultured human myotubes and right panel (C) shows a representative fluorescence image of cultured human myotubes with cells exposed to human serum-derived EVs loaded with Cy3-miR-136-3p. Cy3 fluorescence (red) is detected in the whole cytoplasm of the human myotubes. (D) Representative fluorescence image of human myotubes exposed to <t>HEK293</t> culture medium with EVs loaded with Cy3-miR-136-3p (red). (E) Representative image of human myotubes exposed to EVs loaded with TexasRed-labeled with a control RNA (orange). Nuclear Hoechst staining is shown in blue. Scale bar = 100 μm. EVs = extracellular vesicles; HEK293 = <t>human</t> <t>embryonic</t> <t>kidney;</t> miR = microRNA; Rel = relative; RNU1A1 = U1 small nuclear RNA.

Human Embryonic Kidney Hek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293+cells/Human+embryonic+kidney+cells%2C+293-hY2-10/pmc12811471-83-0-8
Average 96 stars, based on 151 article reviews

human embryonic kidney hek293 cells - by Bioz Stars, 2026-10

96/100 stars

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1) Product Images from "Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle"

Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle

Journal: Journal of Sport and Health Science

doi: 10.1016/j.jshs.2025.101091

Figure Legend Snippet: MiR-136-3p content in cell culture medium and uptake of extracellular miR-136-3p into cultured myotubes. MiRNA content in (A) human myotubes and (B) human pancreatic islets culture media. Results were first normalized using RNU1A1 and then presented in relation to miR-23a-3p content for n = 4 different donors for myotubes cultures and n = 4 donors for human islets. Left panel (C) shows bright-field image of cultured human myotubes and right panel (C) shows a representative fluorescence image of cultured human myotubes with cells exposed to human serum-derived EVs loaded with Cy3-miR-136-3p. Cy3 fluorescence (red) is detected in the whole cytoplasm of the human myotubes. (D) Representative fluorescence image of human myotubes exposed to HEK293 culture medium with EVs loaded with Cy3-miR-136-3p (red). (E) Representative image of human myotubes exposed to EVs loaded with TexasRed-labeled with a control RNA (orange). Nuclear Hoechst staining is shown in blue. Scale bar = 100 μm. EVs = extracellular vesicles; HEK293 = human embryonic kidney; miR = microRNA; Rel = relative; RNU1A1 = U1 small nuclear RNA.

Techniques Used: Cell Culture, Fluorescence, Derivative Assay, Labeling, Control, Staining

NRDC is a direct target of miR-136-3p in human myotubes. Skeletal muscle NRDC mRNA is responsive to training and inactivity. (A) Tissue mRNA expression of NRDC from the Human Protein Atlas database showing enriched expression of NRDC in human skeletal muscle. (B) The miR-136-3p target site in the NRDC gene is highly conserved in mammals. (C) Luciferase activity in HEK293 cells co-transfected the NRDC 3’UTR and miR-136-3p with or without anti-miR136-3p inhibitors. miR-136-3p transfection downregulates NRDC (D) mRNA and (E) representative image of protein abundance in human myotubes. (F) Publicly available data ( GSE14413 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 6 weeks of endurance training ( n = 8). (G) Publicly available data ( GSE120862 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 2 months of aerobic training ( n = 10). (H) Publicly available data ( GSE14901 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 14 days of immobilization ( n = 24). * p < 0.05, ** p < 0.005. GSE = gene set enrichment; HEK293 = human embryonic kidney; miR = microRNA; NC = negative control; NRDC = nardilysin convertase; nTPM = normalized transcripts per million; si NRDC = small interfering RNA of NRDC ; UTR = untranslated region.

Figure Legend Snippet: NRDC is a direct target of miR-136-3p in human myotubes. Skeletal muscle NRDC mRNA is responsive to training and inactivity. (A) Tissue mRNA expression of NRDC from the Human Protein Atlas database showing enriched expression of NRDC in human skeletal muscle. (B) The miR-136-3p target site in the NRDC gene is highly conserved in mammals. (C) Luciferase activity in HEK293 cells co-transfected the NRDC 3’UTR and miR-136-3p with or without anti-miR136-3p inhibitors. miR-136-3p transfection downregulates NRDC (D) mRNA and (E) representative image of protein abundance in human myotubes. (F) Publicly available data ( GSE14413 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 6 weeks of endurance training ( n = 8). (G) Publicly available data ( GSE120862 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 2 months of aerobic training ( n = 10). (H) Publicly available data ( GSE14901 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 14 days of immobilization ( n = 24). * p < 0.05, ** p < 0.005. GSE = gene set enrichment; HEK293 = human embryonic kidney; miR = microRNA; NC = negative control; NRDC = nardilysin convertase; nTPM = normalized transcripts per million; si NRDC = small interfering RNA of NRDC ; UTR = untranslated region.

Techniques Used: Expressing, Luciferase, Activity Assay, Transfection, Quantitative Proteomics, Negative Control, Small Interfering RNA

Negative Control:

Article Title: Artificial Intelligence and Virtual Screening to Identify Small Molecules Inhibiting Transglutaminase 2 for Investigation and Treatment of Breast Cancer.
Article Snippet: The cells were cultured in high‐glucose Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher Scientific, Monza, Italy) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 2 mM L‐glutamine, and antibiotics (50 U/mL peni cillin and 50 μg/mL streptomycin, Sigma–Aldrich Merck). .. HEK293 cells (ATCC CRL‐1573; RRID:CVCL0045) were used as a negative control because they express negligible or very low levels of TG2. .. These cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F‐12 (DMEM/F‐12; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL strepto mycin.

Cell Culture:

Article Title: APC/C−CDH1 suppresses AROS-mediated protection against DNA damage-induced senescence by ubiquitination
Article Snippet: .. HEK293 cells (American Type Culture Collection [ATCC]) were cultured in Dulbecco’s modified Eagle’s medium. .. A549 cells (Korean Cell Line Bank) were cultured in RPMI-1640 medium (WELGENE, Inc.) containing 10% fetal bovine serum and a 1% antibiotic–antimycotic mix (Thermo Fisher Scientific).

Article Title: A bifunctional actuator to correct loss-of-function deficits in cardiac voltage-gated sodium channels
Article Snippet: FixR-Nb-Otud1, the catalytically inactive FixR-Nb-Otud1 C320S mutant, FixR-Nb-Otud4, and FixR-Nb-usp21 were purchased as gene fragments from Twist Biosciences and ligated into the Eco RI and Bam HI sites of an enhanced GFP (eGFP)–pIRES2 vector. .. HEK293 cells (ATCC CRL1573) were cultured in 60-mm dishes on glass coverslips and transfected using the Ca 2+ -phosphate method. ..

Article Title: Heterotrimeric G proteins containing Gβ 1 γ 2 subunits exhibit subtype-specific mobility differences in live cells
Article Snippet: .. CHO-K1 cells (85051005 ECACC) and HEK293 cells (CRL-1573 ATCC) were cultured in F-12K and DMEM medium, respectively, supplemented with 10% Fetal Bovine Serum and an antibiotic/antimycotic mixture at 37 °C and 5% CO 2 . ..

Article Title: Heterotrimeric G proteins containing Gβ1γ2 subunits exhibit subtype-specific mobility differences in live cells.
Article Snippet: .. CHO-K1 cells (85051005 ECACC) and HEK293 cells (CRL-1573 ATCC) were cultured in F-12K and DMEM medium, respectively, supplemented with 10% Fetal Bovine Serum and an antibiotic/antimycotic mixture at 37 °C and 5% CO 2 . ..

Modification:

Article Title: Defining PISD mislocalization as a novel mechanism of mitochondrial damage in Coxsackievirus B3 infection.
Article Snippet: .. HeLa and HEK293 cells (American Type Culture Collection, Manassas, VA, USA; TIB-71CCL-2 and CRL-1573) were maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% FBS and 100 μg/mL penicillin–streptomycin. .. For infection experiments, cells were exposed either to PBS as a sham control (Sigma-Oakville, Oakville, ON, Canada; D8537) or to CVB3 (Kandolf strain, kindly provided by Reinhard Kandolf, University of Tübingen, Tübingen, Germany).

Glass:

Transfection:

Membrane:

Article Title: Development of a random background to understand ligand optimization.
Article Snippet: Relative fluorescence units were exported and analysed using GraphPad Prism 10.0 to calculate IC50 values, from which Ki values were derived using the Cheng–Prusoff equation. .. CB2 receptor-binding assays were performed using membrane preparations from HEK293 cells (ATCC CRL-1573) stably expressing human CB2, following previously published methods67,68. ..

Stable Transfection:

Expressing:

Image Search Results

Journal: Journal of Sport and Health Science

Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle

doi: 10.1016/j.jshs.2025.101091

Figure Lengend Snippet: MiR-136-3p content in cell culture medium and uptake of extracellular miR-136-3p into cultured myotubes. MiRNA content in (A) human myotubes and (B) human pancreatic islets culture media. Results were first normalized using RNU1A1 and then presented in relation to miR-23a-3p content for n = 4 different donors for myotubes cultures and n = 4 donors for human islets. Left panel (C) shows bright-field image of cultured human myotubes and right panel (C) shows a representative fluorescence image of cultured human myotubes with cells exposed to human serum-derived EVs loaded with Cy3-miR-136-3p. Cy3 fluorescence (red) is detected in the whole cytoplasm of the human myotubes. (D) Representative fluorescence image of human myotubes exposed to HEK293 culture medium with EVs loaded with Cy3-miR-136-3p (red). (E) Representative image of human myotubes exposed to EVs loaded with TexasRed-labeled with a control RNA (orange). Nuclear Hoechst staining is shown in blue. Scale bar = 100 μm. EVs = extracellular vesicles; HEK293 = human embryonic kidney; miR = microRNA; Rel = relative; RNU1A1 = U1 small nuclear RNA.

Article Snippet: Human embryonic kidney (HEK293) cells were obtained from American Type Culture Collection (ATCC) and cultured in high-glucose (4.5 g/L) Dulbecco's Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% (vol/vol) FBS.

Techniques: Cell Culture, Fluorescence, Derivative Assay, Labeling, Control, Staining

Journal: Journal of Sport and Health Science

Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle

doi: 10.1016/j.jshs.2025.101091

Figure Lengend Snippet: NRDC is a direct target of miR-136-3p in human myotubes. Skeletal muscle NRDC mRNA is responsive to training and inactivity. (A) Tissue mRNA expression of NRDC from the Human Protein Atlas database showing enriched expression of NRDC in human skeletal muscle. (B) The miR-136-3p target site in the NRDC gene is highly conserved in mammals. (C) Luciferase activity in HEK293 cells co-transfected the NRDC 3’UTR and miR-136-3p with or without anti-miR136-3p inhibitors. miR-136-3p transfection downregulates NRDC (D) mRNA and (E) representative image of protein abundance in human myotubes. (F) Publicly available data ( GSE14413 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 6 weeks of endurance training ( n = 8). (G) Publicly available data ( GSE120862 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 2 months of aerobic training ( n = 10). (H) Publicly available data ( GSE14901 ) showing NRDC mRNA expression in human skeletal muscle of healthy young participants after 14 days of immobilization ( n = 24). * p < 0.05, ** p < 0.005. GSE = gene set enrichment; HEK293 = human embryonic kidney; miR = microRNA; NC = negative control; NRDC = nardilysin convertase; nTPM = normalized transcripts per million; si NRDC = small interfering RNA of NRDC ; UTR = untranslated region.

Techniques: Expressing, Luciferase, Activity Assay, Transfection, Quantitative Proteomics, Negative Control, Small Interfering RNA

Adaptable expression ratios of the creatine transporters CRT1 and MCT12 in HEK293 SLC6A8 −/− cells (A) Scheme of the CRT1 3xHA -P2A-K n -MCT12 2xmyc construct. (B) Immunoblot analysis of CRT1 3xHA -P2A-K n -MCT12 2xmyc . (C) Ratio of MCT12 2xmyc and CRT1 3xHA expression as determined by immunoblotting ( n = 5). Data are represented as mean ± SEM. (D) Immunoblot analysis of CRT1 3xHA -P2A-K n -MCT12 2xmyc . (E) Ratio of MCT12 2xmyc and CRT1 3xHA expression as determined by immunoblotting ( n = 4). Data are represented as mean ± SEM. See also .

Journal: iScience

Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

doi: 10.1016/j.isci.2026.117719

Figure Lengend Snippet: Adaptable expression ratios of the creatine transporters CRT1 and MCT12 in HEK293 SLC6A8 −/− cells (A) Scheme of the CRT1 3xHA -P2A-K n -MCT12 2xmyc construct. (B) Immunoblot analysis of CRT1 3xHA -P2A-K n -MCT12 2xmyc . (C) Ratio of MCT12 2xmyc and CRT1 3xHA expression as determined by immunoblotting ( n = 5). Data are represented as mean ± SEM. (D) Immunoblot analysis of CRT1 3xHA -P2A-K n -MCT12 2xmyc . (E) Ratio of MCT12 2xmyc and CRT1 3xHA expression as determined by immunoblotting ( n = 4). Data are represented as mean ± SEM. See also .

Article Snippet: Human: HEK293 Lenti-X , Takara , Cat # 632180.

Techniques: Expressing, Construct, Western Blot, Analysis

Relative cell surface expression of the creatine transporters CRT1 and MCT12 in HEK293 SLC6A8 −/− cells (A) Scheme of the CRT1 eGFP -P2A-K 0 -MCT12 mCherry (a) and MCT12 mCherry -P2A-K 0 -CRT1 eGFP construct (b). (B) Ratio of mCherry and eGFP expression in eGFP-P2A-K 0 -mCherry (K 0 , as a control), CRT1 eGFP -P2A-K 0 -MCT12 mCherry , and MCT12 mCherry -P2A-K 0 -CRT1 eGFP constructs as determined by flow cytometry ( n = 6). Data are represented as mean ± SEM. (C) Live-cell confocal microscopy of HEK293 SLC6A8 −/− cells expressing CRT1 eGFP -P2A-K 0 -MCT12 mCherry and MCT12 mCherry -P2A-K 0 -CRT1 eGFP . The plasma membrane was stained with CellMask Deep Red. Scale bars, 20 μm. (D) Analysis of cell surface expression of CRT1 eGFP and MCT12 mCherry . n = 30 (CRT1 eGFP -P2A-K 0 -MCT12 mCherry ); n = 28 (MCT12 mCherry -P2A-K 0 -CRT1 eGFP ).

Journal: iScience

Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

doi: 10.1016/j.isci.2026.117719

Figure Lengend Snippet: Relative cell surface expression of the creatine transporters CRT1 and MCT12 in HEK293 SLC6A8 −/− cells (A) Scheme of the CRT1 eGFP -P2A-K 0 -MCT12 mCherry (a) and MCT12 mCherry -P2A-K 0 -CRT1 eGFP construct (b). (B) Ratio of mCherry and eGFP expression in eGFP-P2A-K 0 -mCherry (K 0 , as a control), CRT1 eGFP -P2A-K 0 -MCT12 mCherry , and MCT12 mCherry -P2A-K 0 -CRT1 eGFP constructs as determined by flow cytometry ( n = 6). Data are represented as mean ± SEM. (C) Live-cell confocal microscopy of HEK293 SLC6A8 −/− cells expressing CRT1 eGFP -P2A-K 0 -MCT12 mCherry and MCT12 mCherry -P2A-K 0 -CRT1 eGFP . The plasma membrane was stained with CellMask Deep Red. Scale bars, 20 μm. (D) Analysis of cell surface expression of CRT1 eGFP and MCT12 mCherry . n = 30 (CRT1 eGFP -P2A-K 0 -MCT12 mCherry ); n = 28 (MCT12 mCherry -P2A-K 0 -CRT1 eGFP ).

Article Snippet: Human: HEK293 Lenti-X , Takara , Cat # 632180.

Techniques: Expressing, Construct, Control, Flow Cytometry, Confocal, Microscopy, Clinical Proteomics, Membrane, Staining, Analysis

Journal: iScience

Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

doi: 10.1016/j.isci.2026.117719

Figure Lengend Snippet:

Article Snippet: Human: HEK293 Lenti-X , Takara , Cat # 632180.

Techniques: Cell-Signaling, Virus, Recombinant, Clinical Proteomics, Membrane, Staining, Cloning, Software

Binding of linvoseltamab and teclistamab to WT or mutated BCMA expressed by engineered HEK293/hCD20 cells. (A) Histogram plots from cell staining with 100nM anti-BCMA clone 19F2 or mIgG isotype control. BCMA is expressed on all engineered cells. (B) Histogram plots from staining with 100nM linvoseltamab, teclistamab, or hIgG isotype control. Linvoseltamab binding to BCMA R27P, P33S, and S30del was comparable to WT BCMA. Teclistamab binding to R27P, S30del, and P34del was reduced. (C) EC 50 values (nM) derived from nonlinear regression based on the cell staining experiment. hIgG, human immunoglobulin G; mIgG, mouse immunoglobulin G; NC, not calculated (EC 50 cannot be calculated from the range of concentrations tested); ND, not determined (no concentration-dependent binding was observed).

Journal: Blood Advances

Article Title: Distinct epitope engagement confers differential activity of linvoseltamab vs teclistamab across BCMA mutations ∗

doi: 10.1182/bloodadvances.2026020381

Figure Lengend Snippet: Binding of linvoseltamab and teclistamab to WT or mutated BCMA expressed by engineered HEK293/hCD20 cells. (A) Histogram plots from cell staining with 100nM anti-BCMA clone 19F2 or mIgG isotype control. BCMA is expressed on all engineered cells. (B) Histogram plots from staining with 100nM linvoseltamab, teclistamab, or hIgG isotype control. Linvoseltamab binding to BCMA R27P, P33S, and S30del was comparable to WT BCMA. Teclistamab binding to R27P, S30del, and P34del was reduced. (C) EC 50 values (nM) derived from nonlinear regression based on the cell staining experiment. hIgG, human immunoglobulin G; mIgG, mouse immunoglobulin G; NC, not calculated (EC 50 cannot be calculated from the range of concentrations tested); ND, not determined (no concentration-dependent binding was observed).

Article Snippet: Human embryonic kidney 293 (HEK293) cells (ATCC CRL-1573) stably expressing human CD20 (hCD20) (MS4A1; M1-P297 of NP_690605.1 ) were generated by transfection with a mammalian expression vector and selected in media (Dulbecco's Modified Eagle Medium, 10% fetal bovine serum, and 1% penicillin-streptomycin; Thermo Fisher Scientific) supplemented with 0.5 mg/mL of G418.

Techniques: Binding Assay, Staining, Control, Derivative Assay, Concentration Assay

Effects of BCMA mutations on linvoseltamab- and teclistamab-mediated Jurkat-NFAT cell activation. Jurkat-NFAT reporter cells cocultured with HEK293/hCD20 cells with WT or mutated BCMA; reporter activity was detected as luminescence. A nontargeting CD3 antibody was used as a control. RLU, relative light units.

Journal: Blood Advances

Article Title: Distinct epitope engagement confers differential activity of linvoseltamab vs teclistamab across BCMA mutations ∗

doi: 10.1182/bloodadvances.2026020381

Figure Lengend Snippet: Effects of BCMA mutations on linvoseltamab- and teclistamab-mediated Jurkat-NFAT cell activation. Jurkat-NFAT reporter cells cocultured with HEK293/hCD20 cells with WT or mutated BCMA; reporter activity was detected as luminescence. A nontargeting CD3 antibody was used as a control. RLU, relative light units.

Techniques: Activation Assay, Activity Assay, Control

Effect of BCMA mutations on T-cell–mediated cytotoxicity against HEK293h/CD20 cells treated with linvoseltamab and teclistamab. HEK293 or OPM-2 myeloma cells were incubated for 3 days with isolated human T cells, with E:T of 12.5 (HEK293) and 5 (OPM-2). Target cell survival was determined by flow cytometry. Percentage survival was normalized to untreated cells. The OPM-2 cell line was used as a comparator because this is an MM cell line that endogenously expresses BCMA. E:T, effector-to-target ratio.

Journal: Blood Advances

Article Title: Distinct epitope engagement confers differential activity of linvoseltamab vs teclistamab across BCMA mutations ∗

doi: 10.1182/bloodadvances.2026020381

Figure Lengend Snippet: Effect of BCMA mutations on T-cell–mediated cytotoxicity against HEK293h/CD20 cells treated with linvoseltamab and teclistamab. HEK293 or OPM-2 myeloma cells were incubated for 3 days with isolated human T cells, with E:T of 12.5 (HEK293) and 5 (OPM-2). Target cell survival was determined by flow cytometry. Percentage survival was normalized to untreated cells. The OPM-2 cell line was used as a comparator because this is an MM cell line that endogenously expresses BCMA. E:T, effector-to-target ratio.

Techniques: Incubation, Isolation, Flow Cytometry

Effect of BCMA-binding interface mutations on primary T-cell activation by linvoseltamab and teclistamab. HEK293 or OPM-2 myeloma cells were incubated for 3 days with isolated human T cells, with E:T of 12.5 (HEK293) and 5 (OPM-2). T-cell activation was determined by flow cytometry based on the percentage of CD8 + T cells expressing CD25. The OPM-2 cell line was used as a comparator because this is an MM cell line that endogenously expresses BCMA.

Journal: Blood Advances

Article Title: Distinct epitope engagement confers differential activity of linvoseltamab vs teclistamab across BCMA mutations ∗

doi: 10.1182/bloodadvances.2026020381

Figure Lengend Snippet: Effect of BCMA-binding interface mutations on primary T-cell activation by linvoseltamab and teclistamab. HEK293 or OPM-2 myeloma cells were incubated for 3 days with isolated human T cells, with E:T of 12.5 (HEK293) and 5 (OPM-2). T-cell activation was determined by flow cytometry based on the percentage of CD8 + T cells expressing CD25. The OPM-2 cell line was used as a comparator because this is an MM cell line that endogenously expresses BCMA.

Techniques: Binding Assay, Activation Assay, Incubation, Isolation, Flow Cytometry, Expressing

The cell line name is indicated on the left side of each expression track.

Journal: Life Science Alliance

Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

doi: 10.26508/lsa.202403105

Figure Lengend Snippet: The cell line name is indicated on the left side of each expression track.

Article Snippet: Human lung adenocarcinoma A549 cell line (ATCC No. CCL-185), human colorectal carcinoma HCT116 cell line (ATCC No. CCL-247), and human kidney embryonic HEK293 cell line (ATCC No. CRL-1573) were purchased from ATCC.

Techniques: Expressing

The cell line and replicate are indicated on the left side of each expression track.

Journal: Life Science Alliance

Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

doi: 10.26508/lsa.202403105

Figure Lengend Snippet: The cell line and replicate are indicated on the left side of each expression track.

Techniques: Expressing

(A) CCK8 assay for survival of A549, HCT116, and HEK293 cells transfected with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2; N = 3. (B) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with SNORA13 vector; N = 4. (C) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with TIGA1 vector; N = 4. (D) Schematic gene structure of GRAS1 GapmeR–resistant construct. (E) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with GRAS1 GapmeR–resistant vector compared with empty vector; N = 4. (F) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with SNORA13 vector compared with empty vector; N = 4. (G) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with TIGA1 vector compared with empty vector; N = 4. Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (**) P ≤ 0.01; (***) P < 0.001 by two-tailed t test.

Journal: Life Science Alliance

Article Title: The noncoding RNA GRAS1 interacts with NKAP and protects against DNA damage and cell death

doi: 10.26508/lsa.202403105

Figure Lengend Snippet: (A) CCK8 assay for survival of A549, HCT116, and HEK293 cells transfected with control GapmeR compared with GRAS1 GapmeR1 or GRAS1 GapmeR2; N = 3. (B) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with SNORA13 vector; N = 4. (C) CCK8 assay for survival of A549 and HCT116 cells transfected with empty vector compared with TIGA1 vector; N = 4. (D) Schematic gene structure of GRAS1 GapmeR–resistant construct. (E) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with GRAS1 GapmeR–resistant vector compared with empty vector; N = 4. (F) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with SNORA13 vector compared with empty vector; N = 4. (G) CCK8 assay for survival of A549 and HCT116 GRAS1 knockdown cells complemented with TIGA1 vector compared with empty vector; N = 4. Error bars represent SD in all panels; (ns) P > 0.05; (*) P ≤ 0.05; (**) P ≤ 0.01; (***) P < 0.001 by two-tailed t test.

Techniques: CCK-8 Assay, Transfection, Control, Plasmid Preparation, Construct, Knockdown, Two Tailed Test

Review

human embryonic kidney hek293 cells (ATCC)

Structured Review

Images

1) Product Images from "Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle"

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Negative Control:

Cell Culture:

Modification:

Glass:

Transfection:

Membrane:

Stable Transfection:

Expressing:

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